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Comprehensive Metabolomics in Mouse Mast Cell Model of Allergic Rhinitis for Profiling, Modulation, Semiquantitative

Akshay Suresh Patil1, Yan Xu1

  • 1Department of Chemistry, Cleveland State University, Cleveland, OH 44115, USA.

Biomolecules
|January 25, 2025
PubMed
Summary

This study reveals key metabolic changes in mast cells during allergic rhinitis, identifying specific metabolites and pathways affected. It also shows how triprolidine and zileuton can reverse these allergic rhinitis metabolic shifts.

Keywords:
UHPLC-QTOF-MS-based untargeted and targeted metabolomicsmast cell modulationpathway analysissemiquantitative analysistherapeutic agent testing

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Area of Science:

  • Immunology and Biochemistry
  • Metabolomics

Background:

  • Allergic rhinitis impacts millions globally, causing significant discomfort and reduced quality of life.
  • Mast cells play a crucial role in allergic responses.
  • Understanding mast cell metabolism is vital for developing effective treatments.

Purpose of the Study:

  • To investigate metabolic alterations in murine mast cells (MC/9) during allergic rhinitis.
  • To identify key metabolites and pathways involved in the allergic response.
  • To evaluate the therapeutic potential of triprolidine and zileuton in reversing these metabolic changes.

Main Methods:

  • Utilized UHPLC-QTOF-MS-based untargeted and targeted metabolomics.
  • Induced allergic rhinitis conditions in murine mast cells (MC/9) using lipopolysaccharide (LPS).
  • Analyzed metabolic profiles and pathway modulations.

Main Results:

  • Identified 44 significantly regulated metabolites, including histamine, leukotrienes, prostaglandins, thromboxanes, and ceramides.
  • Highlighted modulation of arachidonic acid, histidine, and sphingolipid metabolism.
  • Demonstrated that triprolidine and zileuton reversed LPS-induced metabolic shifts, with distinct pathway targets.

Conclusions:

  • Metabolomics is a valuable tool for understanding allergic rhinitis pathophysiology.
  • Triprolidine and zileuton show promise in reversing allergic rhinitis-associated metabolic dysregulation.
  • This study provides a model for assessing anti-allergic agent efficacy, aiding in the development of novel treatments.