Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

7.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.3K
Anatomy of the Eyeball01:20

Anatomy of the Eyeball

5.9K
The eye is a spherical, hollow structure composed of three tissue layers. The outer layer — the fibrous tunic, comprises the sclera — a white structure — and the cornea, which is transparent. The sclera encompasses some of the ocular surface, most of which is not visible. However, the 'white of the eye' is distinctively visible in humans compared to other species. The cornea, a clear covering at the front of the eye, enables light penetration. The eye's middle...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A novel OPHN1 variant associated with cyclic strabismus but in the absence of OPHN1 syndrome.

Scientific reports·2026
Same author

Identification of gradual aging and late-onset aging markers using male African turquoise killifish.

Scientific reports·2026
Same author

SAMD4 represses VGLL4 mRNA to activate TEAD and promote cancer progression.

Oncogene·2026
Same author

Left Subclavian Artery Revascularization in Aortic Arch Replacement: Comparative Patency of In Situ Reconstruction and Extra-Anatomic Bypass.

European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery·2026
Same author

Crystal and Cryo-EM structure of PPL, a novel hexameric R-type lectin from the poisonous mushroom Pleurocybella porrigens.

Glycobiology·2025
Same author

Region-specific roles of stress-activated protein kinase MKK7 in the developing and maturing murine brain.

Scientific reports·2025

Related Experiment Video

Updated: May 31, 2025

4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis
07:26

4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis

Published on: May 26, 2021

3.4K

Sonic Hedgehog Determines Early Retinal Development and Adjusts Eyeball Architecture.

Noriyuki Azuma1,2, Keiko Tadokoro3, Masao Yamada3

  • 1Department of Ophthalmology and Laboratory for Visual Science, National Centre for Child Health and Development, Tokyo 157-8535, Japan.

International Journal of Molecular Sciences
|January 25, 2025
PubMed
Summary

Sonic hedgehog (Shh) signaling from the face influences eye development and fovea formation. Shh mutations can lead to eye malformations, impacting visual acuity.

Keywords:
Pax6Sonic hedgehogeye architecturefoveaholoprosencephalyretinal development

More Related Videos

Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish
07:40

Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish

Published on: April 5, 2021

2.7K
Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy
09:16

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Published on: January 30, 2014

11.0K

Related Experiment Videos

Last Updated: May 31, 2025

4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis
07:26

4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis

Published on: May 26, 2021

3.4K
Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish
07:40

Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish

Published on: April 5, 2021

2.7K
Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy
09:16

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Published on: January 30, 2014

11.0K

Area of Science:

  • Developmental biology
  • Ophthalmology
  • Genetics

Background:

  • Vertebrate eyes initially form laterally but adapt for forward vision, a process with an unknown molecular basis.
  • Mutations in Sonic hedgehog (Shh) are linked to holoprosencephaly and altered eye positioning, but facial Shh signaling's role in visual development is unclear.

Purpose of the Study:

  • To investigate the role of Sonic hedgehog (Shh) signaling in vertebrate eye positioning and fovea development.
  • To identify the molecular mechanisms by which Shh influences retinal differentiation and eyeball architecture.

Main Methods:

  • Identified novel Shh gene mutations in patients with dislocated fovea.
  • Performed functional assays to assess Shh's effect on downstream gene expression (Patched, Gli, Pax6).
  • Utilized chick embryo models to study gain- and loss-of-function effects of Shh on retinal development.

Main Results:

  • Shh signaling was found to upregulate Patched and Gli, and downregulate Pax6, with mutations altering these activities.
  • Gain of Shh function in chick embryos inhibited retinal development and eyeball growth.
  • Loss of Shh function promoted retinal development and eyeball growth.

Conclusions:

  • Facial Shh signaling plays a crucial role in controlling neural retina differentiation in a position-specific manner.
  • This Shh-mediated control is essential for the correct formation and positioning of the fovea, impacting visual acuity.