Related Experiment Video
Updated: Aug 21, 2026

Ganglioside Extraction, Purification and Profiling
Published on: March 12, 2021
Expression of Mono- and Dimeric Ganglioside-Binding Lectins and Their Cellular Functions
Kenichi Kamata1,2, Marco Gerdol3, Imtiaj Hasan4,5
1Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Tokyo, Japan.
None:
In the 2020s, a novel R-type lectin family with β-trefoil folding was found in Mytilidae (phylum Mollusca). SeviL, as described in this chapter, is the lectin discovered from Mytilisepta virgata, which is habituated in the intertidal community in the northwestern Pacific Ocean. SeviL is bound to the glycan moiety of gangliosides such as asialo-GM1 (GA1: Galβ1-3GalNAcβ1-4Galβ1-4Glc), GM1b (Neu5Acα2-3Galβ1-3GalNAcβ1-4Galβ1-4Glc), and SSEA-4 (Galβ1-3GalNAcβ1-3Galα1-4Galβ1-4Glcβ1), leading to apoptosis of cancer cells and M1 macrophage polarization. We describe how to prepare dimeric and monomeric SeviLs, which are valuable in studying the function of gangliosides in terms of glycan recognition. It also introduces how to apply lectins to ganglioside studies on concentration-dependent growth inhibition and cell differentiation.
More Related Videos
10:00Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
Published on: November 8, 2016
11:10Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
Published on: June 29, 2016
Related Concept Videos
Immunoglobulin-like Cell Adhesion Molecules
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Selectins
Glycocalyx and its Functions
Components of...
Types of Signaling Molecules
Ligand-Gated Ion Channel Receptor: Gating Mechanism