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LeafJ: An ImageJ Plugin for Semi-automated Leaf Shape Measurement
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Segment Any Leaf 3D: A Zero-Shot 3D Leaf Instance Segmentation Method Based on Multi-View Images.

Yunlong Wang1, Zhiyong Zhang1

  • 1School of Electronic and Communication Engineering, Sun Yat-sen University, Shenzhen 518000, China.

Sensors (Basel, Switzerland)
|January 25, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a novel zero-shot 3D leaf segmentation method for plant phenotyping. It accurately characterizes plant morphology using RGB sensors and advanced AI, enabling precise genetic analysis.

Keywords:
RGB sensorsinstance segmentationmulti-view stereoplant phenotypingzero-shot segmentation

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Area of Science:

  • Plant Science
  • Computer Vision
  • Genetics

Background:

  • Precise plant phenotypic analysis is crucial for understanding genotype-phenotype relationships.
  • Existing methods struggle with plant morphological diversity and require extensive annotated 3D data.
  • Generalizability across species and robustness to variations remain significant challenges.

Purpose of the Study:

  • To develop a zero-shot 3D leaf instance segmentation method for plant phenotyping.
  • To overcome limitations of current methods in handling plant morphological variability and data requirements.
  • To enable accurate 3D characterization of plant phenotypes using readily available RGB sensors.

Main Methods:

  • A multi-view strategy extends the 2D Segment Anything Model (SAM) to 3D.
  • 3D plant point clouds are reconstructed from RGB image sequences using multi-view stereo.
  • High-Quality SAM (HQ-SAM) performs 2D leaf segmentation, mapped to 3D point clouds.
  • An incremental fusion method aggregates multi-view segmentations based on confidence scores.

Main Results:

  • The method achieved point-level precision, recall, and F1 scores exceeding 0.9 on a peanut seedling dataset.
  • Object-level mean Intersection over Union (mIoU) and precision surpassed 0.75.
  • Demonstrated state-of-the-art segmentation quality with zero-shot capability and generalizability.

Conclusions:

  • The proposed method offers a robust and generalizable solution for 3D leaf instance segmentation.
  • It significantly advances plant phenotyping by enabling precise morphological characterization without extensive annotated data.
  • The approach holds substantial potential for accelerating genetic studies and crop improvement programs.