Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNAse I-actin complex: an immunological study.

M Boyer, C Roustan, Y Benyamin

    Bioscience Reports
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    2024 recommendations for the therapeutic management of anogenital warts.

    Annales de dermatologie et de venereologie·2026
    Same author

    Human Papilloma Virus Positive Oropharyngeal Squamous Cell Carcinoma and the Immune System: Pathogenesis, Immunotherapy and Future Perspectives.

    International journal of molecular sciences·2024
    Same author

    Validated French translation of the ESIT-SQ standardized tinnitus screening questionnaire.

    European annals of otorhinolaryngology, head and neck diseases·2023
    Same author

    Pemetrexed plus platinum with or without pembrolizumab in patients with previously untreated metastatic nonsquamous NSCLC: protocol-specified final analysis from KEYNOTE-189.

    Annals of oncology : official journal of the European Society for Medical Oncology·2021
    Same author

    Hand Washing Observations in Fast-Food and Full-Service Restaurants: Results from the 2014 U.S. Food and Drug Administration Retail Food Risk Factors Study.

    Journal of food protection·2021
    Same author

    SARS-CoV-2 antibody responses in patients with acute leukaemia.

    Leukemia·2020
    Same journal

    Expression of Concern: Exosomes derived from circRNA Rtn4-modified BMSCs attenuate TNF-α-induced cytotoxicity and apoptosis in murine MC3T3-E1 cells by sponging miR-146a.

    Bioscience reports·2026
    Same journal

    Evaluation of correlations between serum and urinary vitamin D metabolites using LC-MS/MS.

    Bioscience reports·2026
    Same journal

    The evolving therapeutic landscape of gut-pancreatic peptide signalling in metabolic disorders: from mono- to multi-agonist therapies.

    Bioscience reports·2026
    Same journal

    A peripheral blood-based approach involving vimentin along with AURKA enabled efficient tracking of elusive Oct4/Sox2-expressing disseminated breast cancer stem cells.

    Bioscience reports·2026
    Same journal

    MCM2-7 proteins promote NF-κB transcriptional activity through cooperative promoter recruitment.

    Bioscience reports·2026
    Same journal

    A comprehensive overview of the biological foundations of ADHD.

    Bioscience reports·2026
    See all related articles

    Antibodies targeting a central actin region disrupt DNAse I binding. This reveals that actin

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Protein Interactions

    Background:

    • Actin is a crucial cytoskeletal protein involved in various cellular processes.
    • DNAse I (Deoxyribonuclease I) is an enzyme that binds to actin, forming a stable complex.
    • Understanding the regulation of actin-DNAse I interaction is key to deciphering actin dynamics.

    Purpose of the Study:

    • To investigate the influence of specific anti-actin antibodies on DNAse I-actin complex formation.
    • To map the regions of actin involved in DNAse I binding.
    • To elucidate the spatial arrangement of actin's N- and C-termini relative to the DNAse I binding site.

    Main Methods:

    • Utilized anti-actin antibody populations targeting different actin regions.
    • Studied the effect of these antibodies on the formation of the DNAse I-actin complex.

    Related Experiment Videos

  • Analyzed the spatial proximity of actin's terminal regions.

    • Main Results:

    • Antibodies specific to the central actin region (amino acids 168-226) significantly affected DNAse I binding.
    • The C- and N-termini of actin were found to be in close proximity on the monomer surface.
    • These terminal regions are spatially distant from the DNAse I binding site.

    Conclusions:

    • The central region of actin (168-226) plays a critical role in mediating DNAse I binding.
    • Actin's N- and C-termini are not directly involved in DNAse I interaction.
    • This structural information provides insights into the molecular mechanism of actin-DNAse I complex formation.