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A CRISPR/Cas12a-based competitive aptasensor for ochratoxin A detection
Fengxi Zhu1,2, Qiang Zhao1,2,3
1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China. qiangzhao@rcees.ac.cn.
A new aptasensor uses CRISPR/Cas12a technology for sensitive ochratoxin A (OTA) detection. This method offers a rapid and selective approach for monitoring OTA contamination in agricultural products, enhancing food safety.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Food Science
Background:
- Ochratoxin A (OTA) contamination in agricultural products poses significant risks.
- There is a need for rapid, sensitive, and selective analytical methods for OTA monitoring.
Purpose of the Study:
- To develop a competitive aptasensor for ochratoxin A detection.
- To utilize CRISPR/Cas12a as a signal amplification system for enhanced sensitivity.
Main Methods:
- A competitive aptasensor format was designed using aptamers for OTA binding.
- CRISPR/Cas12a system was employed for signal amplification via DNA reporter cleavage.
- Streptavidin-biotin interactions were used to link aptamer binding to Cas12a activation.
Main Results:
- The aptasensor demonstrated sensitive OTA detection with a linear range of 0.5 nM to 62.5 nM.
- A low detection limit of 0.5 nM was achieved for OTA.
- The method exhibited high selectivity and anti-interference capabilities in complex matrices.
Conclusions:
- The CRISPR/Cas12a-based competitive aptasensor provides a simple and sensitive platform for OTA detection.
- This technology holds significant promise for improving food security monitoring and ensuring the safety of agricultural products.
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