The bacterial composition signatures of perianal abscess and origin of infecting microbes
Song Han1, Wenya Su2, Kefeng Fan3
1Department of Anorectal Surgery, Cheeloo College of Medicine, Qilu Hospital (Qingdao), Qingdao, China.
Background:
Perianal abscess is a common anal condition primarily caused by bacterial infections, yet the precise origins of these infecting bacteria remain unclear. Understanding the distinct microbial signatures associated with periaabscesses is crucial for provide fresh ideas for disease prevention.
Materials And Methods:
Samples of anal skin, feces, and abscesses were collected from a cohort of 75 patients diagnosed with perianal abscesses. The microbial composition at each site was analyzed using 16S rRNA gene sequencing to characterize the bacterial communities present.
Results:
Analysis using MaAslin2 revealed distinct bacterial compositional signatures among the anal skin, feces, and abscess samples. Alpha diversity analysis indicated significant differences in bacterial diversity between abscesses, anal skin, and feces, with abscesses showing lower diversity compared to higher richness in feces. Biomarker analysis highlighted key taxa such as Bacteroides and Escherichia-Shisgella in fecal samples, and Staphylococcus and Corynebacterium in anal skin samples. The analysis of abscess samples suggested that the bacterial composition may originate from the skin, feces, and potentially other sources.
Insights
Perianal abscesses are bacterial infections with unclear origins. This study identified distinct bacterial signatures in abscesses, anal skin, and feces, suggesting potential sources for infection and informing prevention strategies.
Area of Science:
- Microbiology
- Gastroenterology
- Infectious Diseases
Background:
- Perianal abscess is a common condition caused by bacterial infections.
- The exact sources of bacteria causing perianal abscesses are not well understood.
- Identifying microbial signatures is key for developing prevention strategies.
Purpose of the Study:
- To investigate the microbial composition of perianal abscesses.
- To compare the bacterial communities in abscesses with those from anal skin and feces.
- To identify potential bacterial sources contributing to perianal abscess formation.
Main Methods:
- Collected samples from anal skin, feces, and abscesses of 75 patients.
- Utilized 16S rRNA gene sequencing to analyze bacterial communities.
- Employed MaAslin2 for compositional analysis and alpha diversity assessment.
Main Results:
- Distinct bacterial signatures were found in abscess, anal skin, and fecal samples.
- Abscesses exhibited lower bacterial diversity compared to feces.
- Key taxa like *Bacteroides* and *Escherichia-Shisgella* were prevalent in feces, while *Staphylococcus* and *Corynebacterium* were found on anal skin.
Conclusions:
- Bacterial composition in perianal abscesses may originate from anal skin and feces.
- Further research into these sources could lead to novel prevention methods.
- Understanding microbial dynamics is crucial for managing perianal abscesses.
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