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Updated: May 30, 2025

Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models
Published on: March 6, 2018
Swine clones: potential application for animal production and animal models
Thaís Naomi Gonçalves Nesiyama1, Juliano Rodrigues Sangalli1, Tiago Henrique Camara De Bem1
1Faculdade de Zootecnia e Engenharia de Alimentos - FZEA, Universidade de São Paulo - USP, Pirassununga, SP, Brasil.
Abstract:
Somatic cell nuclear transfer (SCNT), or cloning, is used to reprogram cells and generate genetically identical embryos and animals. However, the cloning process is inefficient, limiting its application to producing valuable animals. In swine, cloning is mainly utilized to produce genetically modified animals. Indeed, recombinant DNA technologies have evolved considerably in recent years, with homologous recombination and gene editing technologies becoming more efficient and capable of recombining both alleles in a single cell. The selection of appropriate cells and their use as nuclear donors for SCNT is the most common method for generating edited and genetically modified animals for commercial and research purposes. This article reviews current applications of swine cloning and shares our personal experiences with the procedure in this species.
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