Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

2.8K
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
2.8K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

3.9K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
3.9K
iPS Cell Differentiation01:22

iPS Cell Differentiation

2.6K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative Analysis of Extracellular Vesicle-Like Particles From Different Processing <i>Gastrodia elata</i> Bl.: Physicochemical Properties, Biosafety, and Neuroprotection Potential as a Functional Food Ingredient.

Food science & nutrition·2026
Same author

Mixing Ratio and Relative Humidity Govern Dimethylamine Uptake on Ammonium Sulfate-Glyoxylic Acid Aerosols: Kinetics and Brown Carbon Formation.

Environmental science & technology·2026
Same author

Baveno Criteria Spare Endoscopies Among Patients With Compensated Cirrhosis Within a Large US Healthcare System.

Gastro hep advances·2026
Same author

Pervasive and programmed nucleosome distortion on single chromatin fibres.

Nature·2026
Same author

Cosolvent-Modulated Donor Preaggregation Enhances Molecular Order in 20% Efficient Bilayer Organic Solar Cells.

ACS applied materials & interfaces·2026
Same author

Comparison of the predictive value of NUTRIC and modified NUTRIC scores for ICU mortality in patients with sepsis: a single-center prospective cohort study.

Scientific reports·2026
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: May 30, 2025

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
12:40

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Published on: May 19, 2018

10.2K

Predicting Metabolic Dysfunction Associated Steatotic Liver Disease Risk Using Patient-Derived Induced Pluripotent

Yuanyuan Qin1, Parth Chhetri1, Elizabeth Theusch1

  • 1Department of Pediatrics, University of California San Francisco.

Biorxiv : the Preprint Server for Biology
|January 27, 2025
PubMed
Summary
This summary is machine-generated.

Metabolic Dysfunction Associated Steatotic Liver Disease (MASLD) can be predicted using a novel test. Patient-derived stem cells show increased fat accumulation, aiding early risk identification for MASLD and MASH.

Keywords:
Functional lipid assessmentMASLD cohortsMASLD prevention strategiesindividual susceptibilitypatient-derived iPSCsprecision medicine

More Related Videos

Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders
10:31

Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders

Published on: February 9, 2022

3.2K
Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
10:30

Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: August 4, 2022

2.8K

Related Experiment Videos

Last Updated: May 30, 2025

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
12:40

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Published on: May 19, 2018

10.2K
Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders
10:31

Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders

Published on: February 9, 2022

3.2K
Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
10:30

Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: August 4, 2022

2.8K

Area of Science:

  • Hepatology
  • Stem Cell Biology
  • Genetics

Background:

  • Metabolic Dysfunction Associated Steatotic Liver Disease (MASLD) is a growing health concern, often reversible in early stages.
  • Early identification of individuals at high risk for MASLD and its progressive form, MASH, is crucial for timely intervention.
  • Previous research indicated that patient-derived induced pluripotent stem cells (iPSCs) with MASLD genetic risk variants accumulate more lipids.

Purpose of the Study:

  • To develop and validate an iPSC-based predictive tool for MASLD risk.
  • To assess the utility of functional lipid accumulation assays in iPSCs for predicting MASLD and MASH development.

Main Methods:

  • Induced pluripotent stem cells (iPSCs) were generated from patients and controls across three diverse cohorts.
  • Oleate-induced intracellular lipid accumulation was quantified in iPSCs.
  • An iPSC-based MASLD risk score was developed using lipid accumulation data and tested for predictive accuracy.

Main Results:

  • MASLD/MASH cases consistently showed higher oleate-induced lipid accumulation in iPSCs compared to controls across all cohorts.
  • The developed iPSC-based risk score demonstrated predictive capabilities, with varying sensitivity and specificity across cohorts (e.g., 75% sensitivity and 100% specificity in the UCSF cohort).
  • Variability in performance may be attributed to differences in disease severity and patient cardiometabolic profiles.

Conclusions:

  • Functional lipid accumulation in subject-derived iPSCs is a promising indicator of MASH development.
  • The iPSC-based risk score shows potential for identifying individuals at risk for MASLD.
  • Further validation in larger, diverse cohorts and exploration of additional cellular phenotypes are necessary to enhance predictive accuracy for MASLD surveillance and prevention.