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Updated: May 2, 2026

Production and Titering of Recombinant Adeno-associated Viral Vectors
Published on: November 27, 2011
Extensive length and homology dependent chimerism in pool-packaged AAV libraries
Jean-Benoît Lalanne1,2, John K Mich3, Chau Huynh1,4
1Department of Genome Sciences, University of Washington, Seattle, WA, USA.
Abstract:
Adeno-associated viruses (AAVs) have emerged as the foremost gene therapy delivery vehicles due to their versatility, durability, and safety profile. Here we demonstrate extensive chimerism, manifesting as pervasive barcode swapping, among complex AAV libraries that are packaged as a pool. The observed chimerism is length- and homology-dependent but capsid-independent, in some cases affecting the majority of packaged AAV genomes. These results have implications for the design and deployment of functional AAV libraries in both research and clinical settings.
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