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Updated: Jul 20, 2026

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods
Published on: September 10, 2016
Development of nutri-functional paneer whey-based kefir drink
Harisha Devi1, Tejinder Pal Singh1, Ruby Siwach2
1Department of Dairy Microbiology, College of Dairy Science and Technology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, 125001 Haryana India.
Paneer whey was successfully transformed into a functional fermented product using kefir culture. This innovative approach offers a sustainable solution for dairy waste management and provides potential health benefits.
Area of Science:
- Food Science
- Microbiology
- Biotechnology
Background:
- Paneer whey is a significant dairy byproduct often posing waste management challenges.
- Developing value-added products from paneer whey can enhance its economic viability and reduce environmental impact.
Purpose of the Study:
- To biotransform paneer whey into a functional fermented product using kefir culture.
- To optimize the formulation for consumer acceptability and nutritional value.
- To evaluate the physicochemical, microbial, and functional properties of the developed product.
Main Methods:
- Nine different formulations of paneer whey were prepared with varying concentrations of Fructooligosaccharides (FOS) and refined sugar.
- The most acceptable formulation (S-8) was selected based on sensory evaluation.
- Nutritional analysis, pH, acidity, lactose content, alcohol, and carbon dioxide levels were determined.
- Antimicrobial and antioxidant activities were assessed.
- Microbial stability was evaluated by checking for E.coli and quantifying lactic acid bacteria and yeast.
Main Results:
- Formulation S-8, containing 1% FOS and 8% refined sugar, was identified as the most acceptable.
- The product contained 44.24 kcal energy, 8.29 g carbohydrates, 1.51 g protein, and 0.52 g fat per 100g.
- Fermentation led to a decrease in pH and lactose content, with a corresponding increase in acidity and ethanol.
- The product exhibited significant antimicrobial and antioxidant activities.
- The fermented paneer whey remained stable for 12 days under refrigeration and met microbiological standards for fermented milk products.
Conclusions:
- Paneer whey can be effectively biotransformed into a palatable and nutritious fermented product using kefir culture.
- The developed paneer whey-based kefir offers a sustainable approach to dairy waste utilization.
- The product demonstrates promising health benefits due to its antioxidant and antimicrobial properties.
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