Related Experiment Video
Updated: May 30, 2025

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
Published on: November 9, 2020
[Exploring protein degradation pathways as therapeutic innovations]
Marie Cornu1, Thomas Lemaitre1, Florian Schwalen2
1Université de Caen Normandie, CERMN UR4258, Boulevard Becquerel, 14000 Caen, France.
Abstract:
The disruption of proteostasis provides a favourable context for the emergence of therapeutic innovations, in particular by exploiting technologies such as the PROTAC (Proteolysis Targeting Chimera) approach. These technologies aim to selectively target proteins involved in various diseases, including cancer and neurodegenerative diseases, by inducing their specific degradation via the ubiquitin-proteasome system. The PROTAC approach opens new opportunities for restoring altered protein homeostasis and modulating the pathological consequences of proteostasis deregulation. In addition, by targeting proteins that are resistant to conventional therapeutic approaches, this new strategy offers a hope of treatment. By degrading target proteins, regardless of their structure or of mutations, the PROTAC approach opens the way to reaching previously inaccessible targets and reducing resistance to treatment.
Related Concept Videos
Regulated Protein Degradation
The Proteasome
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
Protein Networks
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Proteins: From Genes to Degradation
Transcription is the synthesis of RNA...
Export of Misfolded Proteins out of the ER
Protein-protein Interfaces

