Exploring the Internal Environmental Changes of Muscle Cells and Apoptotic Phase of Mitochondria in Dry-Cured Loin...

Traditional dry-curing methods have a long cycle time and low efficiency, resulting in the inconsistent quality of dry-cured ham. By applying electrical stimulation (ES) technology in the dry-curing process, it was found that ES affected mitochondrial apoptosis by modulating the intracellular environment of muscle cells, which, in turn, enhanced the quality of dry-cured pork loin. Specifically, ES accelerated glycogen and ATP depletion, which led to a rapid decline in pH. Meanwhile, compared to the control group (CK), the activities of Na⁺-K⁺-ATPase and Ca²⁺-ATPase in the ES group (ES) increased by 160% and 124%, respectively, leading to the generation of H⁺ gradient and Ca²⁺ overload in mitochondria, which in turn triggered mitochondrial apoptosis and increased the apoptosis rate by 259%. In addition, Western blot analysis showed that ES promoted the Desmin and Troponin-T degradation levels. This study highlights the advantages of ES in dry-cured ham processing, which is expected to be a precise regulation technology.