Mouse Model of Lipopolysaccharide (LPS)-Induced Pulpitis

Area of Science:

• Oral Biology
• Dental Research
• Inflammation Models

Background:

• Pulpitis is a prevalent oral disease requiring effective animal models for research.
• Existing models using dogs and pigs have limitations, including complex procedures and gene editing difficulties.
• Previous mouse models often involved direct pulp exposure, limiting research scope.

Purpose of the Study:

• To establish a simplified and effective mouse model for studying pulpitis.
• To utilize lipopolysaccharide (LPS) to induce pulpitis in mice.
• To assess the model's ability to replicate different stages of pulpitis for research purposes.

Main Methods:

• Established a mouse model by accessing the pulp cavity, applying lipopolysaccharide (LPS), and filling the tooth.
• Utilized C57BL/6 mice for the model development.
• Histopathological analysis was performed at 1, 10, and 30 days post-surgery to evaluate inflammatory responses and tissue changes.

Main Results:

• One day post-surgery, the model showed necrotic tissues, inflammatory exudate, and neutrophil infiltration, indicative of early-stage pulpitis.
• Ten days post-surgery, observations included fibroblast changes and dilated blood vessels, reflecting middle and later stages of pulpitis.
• Thirty days post-surgery, significant necrosis in the coronal and root pulp indicated the end stage of pulpitis, validating the model's progression.

Conclusions:

• The developed mouse model is easy to establish and accurately mimics pulpitis progression.
• The model exhibits clear inflammatory phenotypes and is compatible with gene editing techniques.
• This LPS-induced mouse model is suitable for basic research on pulpitis, offering significant practical value.