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Liver Regeneration

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The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
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Related Experiment Video

Updated: May 30, 2025

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

Published on: March 14, 2020

Decoding liver fibrogenesis with single-cell technologies.

Tingting Zhou¹, Musunuru Kiran², Kathy O Lui³

¹CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

|January 28, 2025

Summary

This summary is machine-generated.

Single-cell RNA sequencing is revolutionizing liver fibrosis research by revealing cellular and molecular details of liver fibrogenesis. This technology uncovers critical changes in liver cells, paving the way for new fibrosis therapies.

Keywords:

fibrotic niche lipid-associated macrophage (LAM)non-parenchymal cells stellakines zonation

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Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

Related Experiment Videos

Last Updated: May 30, 2025

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

Published on: March 14, 2020

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Published on: February 18, 2017

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

Area of Science:

Hepatology and immunology
Molecular biology and genetics

Background:

Liver fibrogenesis, a complex process driving chronic liver disease, lacks effective therapies.
Current understanding of liver fibrogenesis mechanisms remains incomplete despite extensive research.

Purpose of the Study:

To review how single-cell RNA sequencing (scRNA-seq) advances the understanding of liver fibrogenesis.
To highlight scRNA-seq discoveries in cell subpopulations, molecular events, and cell interactions relevant to liver fibrosis.

Main Methods:

Review of recent single-cell RNA sequencing studies on liver fibrosis.
Analysis of scRNA-seq data characterizing hepatocytes, liver sinusoidal endothelial cells, myofibroblasts, and macrophages.

Main Results:

scRNA-seq provides high-resolution insights into cellular alterations during liver fibrogenesis.
Discoveries include novel disease-relevant cell subpopulations and molecular events.
Key cell interactions influencing fibrosis progression have been elucidated.

Conclusions:

scRNA-seq is transforming the study of liver fibrogenesis.
Understanding these pathophysiological changes is crucial for developing effective fibrosis treatments.