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Developmental and environmental effects on VTC2-dependent leaf ascorbate accumulation and functions.

Sandrine Kappel1, Maureen J Frieboes1, Ryo Yokoyama1

  • 1Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, D-14476 Potsdam, Germany.

Journal of Experimental Botany
|January 28, 2025
PubMed
Summary
This summary is machine-generated.

Loss of the VTC2 gene depletes ascorbate in mature plant leaves, limiting photoprotection and causing bleaching under fluctuating light. Environmental factors influence this ascorbate deficiency and its impact on plant stress response.

Keywords:
Acclimationascorbatefluctuating environmentsleaf agenon-photochemical quenchingphotosynthesis

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Area of Science:

  • Plant physiology
  • Molecular biology
  • Biochemistry

Background:

  • Plants face photo-oxidative stress from fluctuating environmental conditions.
  • Ascorbate (vitamin C) is crucial for plant stress tolerance, scavenging reactive oxygen species.
  • Ascorbate facilitates non-photochemical quenching (NPQ), a key photoprotective mechanism dependent on zeaxanthin.

Purpose of the Study:

  • To investigate the role of the VTC2 gene in ascorbate biosynthesis and its impact on NPQ.
  • To understand how developmental and environmental factors affect VTC2-dependent ascorbate accumulation and function.

Main Methods:

  • Utilized a newly identified vtc2 allele in plants.
  • Assessed ascorbate levels and NPQ induction under controlled and natural environmental conditions.
  • Monitored zeaxanthin accumulation and leaf bleaching phenotypes.

Main Results:

  • Loss of VTC2 function primarily depleted ascorbate in mature leaves, impairing NPQ in this tissue.
  • Fluctuating light and controlled climate partially mitigated the slow NPQ induction in vtc2 mutants, correlating with higher zeaxanthin levels.
  • Ascorbate-deficient mature leaves of vtc2 mutants bleached under natural fluctuating light and temperature conditions.

Conclusions:

  • VTC2 is essential for maintaining ascorbate levels in mature leaves, directly impacting NPQ capacity.
  • Plant development and environmental conditions significantly modulate VTC2-mediated ascorbate accumulation and its protective functions.
  • VTC2-dependent ascorbate is critical for preventing photo-oxidative damage in mature leaves under dynamic environmental stress.