Screening, identification, and application of anaerobic ammonia oxidizing bacteria in activated sludge systems: A...

Anaerobic ammonium oxidation (Anammox) has garnered significant attention due to its ability to eliminate the need for aeration and supplementary carbon sources in biological nitrogen removal process, relying on the capacity of anaerobic ammonium oxidizing bacteria (AnAOB) to directly convert ammonium and nitrite nitrogen into nitrogen gas. This review consolidates the latest advancements in AnAOB research, outlining the mechanisms and enzymatic processes of Anammox, and summarizing the molecular biological techniques used for studying AnAOB, such as 16s rRNA sequencing, qPCR, and metagenomic sequencing. Additionally, it also overviews the currently identified AnAOB species and their distinct metabolic traits, while consolidating strategies to improve their performance. It further delineates coupled processes that utilize Anammox technology, offering practical insights for process selection. Eventually, the review concludes by suggesting future research directions and highlighting critical areas for further investigation. This review serves as a theoretical reference for the enrichment and cultivation of AnAOB, environmental impact management, and the selection of effective treatment processes.