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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Updated: May 30, 2025

Extraction of High Molecular Weight DNA from Microbial Mats

Extraction of High Molecular Weight DNA from Microbial Mats

Published on: July 7, 2011

Optimized DNA extraction protocol for Staphylococcus aureus strains utilizing liquid nitrogen.

Mariana B Galeano¹, Stefania A Robaldi¹, Tania B Gordillo¹

¹Instituto De Química Biológica de la Facultad de Ciencias Exactas y Naturales-CONICET, Buenos Aires, Argentina; Departamento de Química Biológica, FCEyN-UBA, Buenos Aires, Argentina.

Revista Argentina De Microbiologia

|January 28, 2025

Summary

This summary is machine-generated.

We developed a cost-effective DNA extraction method for Staphylococcus aureus using liquid nitrogen and mechanical lysis. This approach avoids expensive enzymes, yielding high-quality DNA suitable for molecular biology applications.

Keywords:

DNA extraction Extracción de DNA Liquid nitrogen lysis Lisis de nitrógeno líquido Staphylococcus aureus

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Last Updated: May 30, 2025

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Published on: July 7, 2011

Removal of Exogenous Materials from the Outer Portion of Frozen Cores to Investigate the Ancient Biological Communities Harbored Inside

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A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

Area of Science:

Microbiology
Molecular Biology
Biochemistry

Background:

DNA extraction is essential for molecular techniques like whole-genome sequencing.
Staphylococcus aureus cell lysis is challenging due to its resistant peptidoglycan layer.
Conventional methods often rely on expensive enzymatic lysis, such as using lysostaphin.

Purpose of the Study:

To develop a reproducible and cost-effective DNA extraction method for Staphylococcus aureus.
To overcome the limitations of traditional lysis techniques.

Main Methods:

A novel lysis approach using liquid nitrogen and mechanical disruption in a mortar was employed.
DNA purification was performed using phenol-chloroform extraction and precipitation.
DNA quality and suitability for downstream applications were validated using Polymerase Chain Reaction (PCR).

Main Results:

The developed method successfully lysed Staphylococcus aureus USA300 and clinical isolates.
High-quality DNA was obtained with suitable concentration and purity for molecular biology.
PCR amplification confirmed the integrity and usability of the extracted DNA.

Conclusions:

This novel method provides an effective, non-enzymatic lysis strategy for Staphylococcus aureus.
The technique is cost-effective and yields DNA suitable for various molecular applications, circumventing the need for lysostaphin.