The regulation of methionine biosynthesis and metabolism in plants and bacteria

The amino acids biosynthetically derived from asparate including methionine are all essential in the diet of monogastric animals. Most of this requirement is met by plant foods. The methionine biosynthetic pathways in plants and bacteria are outlined and compared. Regulation in bacterial systems is by a combination of repression and feedback inhibition whereas in plants repression is unimportant. Several enzymes in the branched pathway to methionine in plants are regulated by feedback inhibition; others are yet to be investigated. In plants may amino acid biosynthetic enzymes are localized in plastids and this is also likely for methionine biosynthesis. Methionine occupies an important position in cellular metabolism where the processes of one-carbon transfer via S-adenosylmethionine, protein synthesis, protein initiation and ethylene synthesis are interlocked. Attempts to increase the levels of free methionine have been made by selecting for plant mutants resistant to lysine plus threonine. One dominant mutation causes elevation of free amino acid levels in vegetative tissues but also has undesirable side-effects. The potential of such approaches is discussed.