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Engineered Cell Membrane-Coated Keratin Nanoparticles Attenuated Intervertebral Disc Degeneration by Remodeling the
Hongyuan Xing1, Run Li1, Zizhan Huang1
1Department of Orthopedic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.
Advanced Healthcare Materials
|January 29, 2025
Summary
Engineered nanoparticles combat intervertebral disc degeneration by protecting nucleus pulposus cells and remodeling extracellular matrix. This multi-targeted approach shows promise for treating degenerative disc disease.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Cell Biology
Background:
- Intervertebral disc degeneration involves complex changes in nucleus pulposus (NP) cells, extracellular matrix (ECM), and biomechanics, leading to degenerative lumbar disorders.
- Current therapies targeting single factors show limited efficacy, highlighting the need for multi-targeted strategies.
Purpose of the Study:
- To develop engineered cell membrane-coated keratin nanoparticles (MKNs) for simultaneous alleviation of NP cell senescence and ECM remodeling.
- To investigate the therapeutic potential of MKNs in treating intervertebral disc degeneration.
Main Methods:
- Synthesized keratin nanoparticles coated with adipose mesenchymal stem cell membranes.
- Evaluated MKN protection of NP cells against oxidative stress-induced mitochondrial damage.
- Assessed MKN effects on mitochondrial metabolism, NP cell senescence, integrin activation, and ECM remodeling.
Main Results:
- MKNs protected mitochondrial integrity and modulated mitochondrial metabolism in NP cells.
- MKNs attenuated NP cell senescence and activated cell-membrane integrins.
- MKNs enhanced NP cell-ECM interactions, promoting ECM anabolism and reducing catabolism.
Conclusions:
- The developed MKNs offer a multi-targeted strategy to counteract the degenerative cycle in intervertebral disc degeneration.
- This approach effectively treats disc degeneration by addressing NP cell senescence and ECM remodeling.
- MKNs demonstrate potential for future clinical applications in treating degenerative disc disease.
Keywords:
antisenescencedisc degenerationextracellular matrixkeratinoxidative stresssalivary acid glycoengineeringMore Related Videos
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