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IS15DIV-flanked composite transposon harboring bla NDM-5 in multidrug-resistant Salmonella Typhimurium

Kaiting Zhao1, Jing Jin2, Yuan Liao2

  • 1Department of Hematology, Lishui People's Hospital, The Sixth Affiliated Hospital of Wenzhou Medical University, Lishui, Zhejiang, China.

Iscience
|January 29, 2025
PubMed

Insights

Mobile genetic elements carrying antibiotic resistance genes transferred between bacteria in a child. This study reveals how multidrug-resistant Salmonella Typhimurium spreads through mobile genetic elements (MGEs).

Area of Science:

  • Microbiology
  • Genetics
  • Public Health

Background:

  • Multidrug-resistant Salmonella Typhimurium is a growing global health threat.
  • Asymptomatic gastrointestinal carriage facilitates the spread of antibiotic-resistant bacteria.
  • Directly observing mobile genetic element (MGE) transfer in vivo is difficult.

Purpose of the Study:

  • To investigate the in vivo transfer of mobile genetic elements (MGEs) harboring the blaNDM-5 gene.
  • To identify the genetic basis of MGE transfer between Escherichia coli and Salmonella Typhimurium in a child.

Main Methods:

  • BLAST analysis to compare plasmid sequences.
  • Conjugation experiments to assess plasmid transferability.

Main Results:

  • Mobile genetic elements (MGEs) carrying blaNDM-5 were transferred from Escherichia coli to Salmonella Typhimurium in a child.
  • The transferred transposon showed high similarity to previously reported plasmids, indicating potential genetic recombination.
  • The transconjugant plasmid was identified as a conjugative IncI1-I(alpha) plasmid carrying blaNDM-5 and sul1 genes.

Conclusions:

  • This study provides direct evidence of MGE transfer in vivo.
  • It elucidates the genetic mechanisms underlying the spread of antibiotic resistance in Salmonella Typhimurium.
  • Findings offer insights into the genetic basis of IS15DIV-flanked composite transposons in Salmonella Typhimurium.
Keywords:
BiochemistryMicrobiologyMultidrug-resistant organisms

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