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Composition and metabolite patterns of caproic acid-producing bacteria during pH-mediated pitmud-Huangshui
Huimin Zhang1, Xiuben Wang1, Li Zhen1
1Engineering Laboratory for Industrial Microbiology Molecular Beeding of Anhui Province, College of Biological Food Engineering, Anhui Polytechnic University, 8 Middle Beijing Road, Wuhu 241000, Anhui Province, People's Republic of China.
Abstract:
The growth and metabolism of caproic acid - producing bacteria (CPBs) in the pit mud (PM) within the Huangshui (HS) are crucial for both the quality of Chinese Nongxiangxing Baijiu (NXXB) fermentation and the aging of the PM. Based on multi-database co-annotation and key affecting factors identification of CPBs, the growth and metabolites pattern of CPBs was studied through the pH-mediated PM-HS co-fermentation. CPBs in PM were mainly from Oscillospiraceae family. The composition and metabolites profiles of CPBs changed with initial pH-adjustment. Lactobacillus (88.61 %-89.41 %) dominated the PM-HS system at an initial pH of 5, with CPBs suppressed. Butyric acid-producing Clostridium (56.18 %-54.53 %, 19.61 %-42.71 %) and CPBs (9.35 %-5.19 %, 65.44 % 50.01 %) co-dominated the PM-HS system with initial pH values of 6 and 7 respectively. This study may help illuminate the role of CPBs in PM aging, facilitating the targeted CPBs-enrichment, unknown CPBs-isolation, and practical regulation of CPBs-bioaugmentation in NXXB ecosystem.
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