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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
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Magnetic beads-based double-stranded DNA fluorescent aptasensor biosensor for deoxynivalenol detection.

Jiajia Meng1, Ruijiao Li1, Xinyue Chen1

  • 1Institute for Agro-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, 1000 Jingqi Road, Shanghai 201403, China.

Food Chemistry
|January 30, 2025
PubMed
Summary

A new fluorescent biosensor using magnetic beads can detect Deoxynivalenol (DON) in cereals. This sensitive method offers rapid analysis and accurate results for ensuring food safety.

Keywords:
AptamerDeoxynivalenolDouble-stranded DNAFluorescent aptasensorMagnetic beans

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Area of Science:

  • Food Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Deoxynivalenol (DON) is a harmful mycotoxin impacting human and animal health.
  • Accurate detection of DON in food commodities is crucial for public health and regulatory compliance.

Purpose of the Study:

  • To develop a simple, sensitive, and selective magnetic beads-based fluorescent biosensor for DON detection.
  • To validate the biosensor's performance in real food samples and compare it with established methods.

Main Methods:

  • Utilized streptavidin-coated magnetic beads functionalized with a DNA complex (biotin-sDNA+FAM-cDNA/AP).
  • Employed the specific binding of DON to the aptamer probe (AP) to release FAM-labeled cDNA (FAM-cDNA).
  • Quantified released FAM-cDNA via fluorescence measurement after magnetic separation.

Main Results:

  • Achieved a linear detection range of 0.1-50 ng/mL for DON with a high correlation coefficient (R² = 0.9972).
  • Demonstrated high sensitivity with a detection limit of 0.033 ng/mL.
  • Validated the method in paddy, wheat, and maize samples, showing good recoveries (95.48-112.48%) and agreement with UPLC-MS/MS.

Conclusions:

  • The developed magnetic beads-based fluorescent aptasensor provides a promising tool for rapid and sensitive DON detection in cereals.
  • The strategy is adaptable for detecting other target molecules by modifying aptamer and cDNA sequences.
  • This offers a valuable approach for food safety monitoring and mycotoxin analysis.