Temperature-driven CsgA-enhanced mutant enable distinct self-assembly

Area of Science:

• Biomaterials Science
• Protein Engineering
• Nanotechnology

Background:

• Functional coatings are crucial in technology, but their efficacy relies on suitable media.
• Developing versatile biomaterials for advanced applications remains a key challenge.

Purpose of the Study:

• To engineer a novel protein-based molecular glue, CsgA-pro, for biotherapy.
• To demonstrate the utility of CsgA-pro as a scaffold for functional nanomaterials.
• To evaluate the therapeutic potential of CsgA-pro-based constructs in wound healing.

Main Methods:

• Engineered a CsgA variant (CsgA-pro) with temperature-dependent fiber formation and molecular glue properties.
• Immobilized CsgA-pro-GFP onto gauze and functionalized with gold nanoparticles (Au NPs).
• Assessed antibacterial activity, cell migration (L929, HUVEC), and wound healing efficacy in vivo.

Main Results:

• CsgA-pro demonstrated a temperature-driven transition, forming protein fibers and acting as a molecular glue.
• Au NP-loaded gauze exhibited potent antibacterial effects.
• Au NP-loaded gauze significantly accelerated wound healing and promoted cell migration.

Conclusions:

• CsgA-pro is a versatile platform for developing advanced functional materials.
• CsgA-pro-based Au NP scaffolds show significant therapeutic potential for wound healing.
• This approach offers broad applicability in biocatalysis, immobilization, and biomedical advancements.