Near Infrared-Mediated Intracellular NADH Delivery Strengthens Mitochondrial Function and Stability in Muscle...

Area of Science:

• Cell Biology
• Mitochondrial Medicine
• Regenerative Medicine

Background:

• Mitochondrial transfer is a promising cell therapy due to low immunogenicity.
• Isolated mitochondria lose function rapidly outside the cell, limiting therapeutic potential.
• Nicotinamide adenine dinucleotide (NADH) is crucial for mitochondrial energy production but has poor cellular uptake.

Purpose of the Study:

• To investigate if NIR-mediated NADH pre-treatment enhances mitochondrial function and stability.
• To develop a photo-mediated method for improved NADH delivery and absorption into cells.
• To evaluate the efficacy of enhanced mitochondria in treating muscle dysfunction.

Main Methods:

• L6 cells were treated with NADH and irradiated with near-infrared (NIR) light.
• Mitochondrial function, energy production, and stability were assessed.
• Enhanced mitochondria (P-MT) were encapsulated in liposomes and delivered to dysfunctional muscle cells.

Main Results:

• NIR-mediated NADH uptake significantly improved mitochondrial energy production and function in L6 cells.
• Enhanced mitochondrial function was maintained after isolation from donor cells.
• Liposome-encapsulated P-MT promoted greater mitochondrial recovery and muscle regeneration compared to conventional mitochondria.

Conclusions:

• NIR-mediated NADH delivery is an effective strategy to enhance mitochondrial function and stability.
• Pre-treated mitochondria (P-MT) demonstrate improved therapeutic potential for mitochondrial disorders.
• This approach offers a novel treatment strategy for muscle degeneration and related conditions.