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Related Experiment Video

Updated: May 30, 2025

Author Spotlight: Elucidating the Dynamics of Mechano-Transduction and Nuclear Agitation in Mouse Oocytes
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Author Spotlight: Elucidating the Dynamics of Mechano-Transduction and Nuclear Agitation in Mouse Oocytes

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Protocol to image, segment, and quantify cortical contractions in maturing mouse oocytes.

Gaëlle Letort1, Elvira Nikalayevich2

  • 1Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR 3738, Université Paris Cité, 25 Rue du Dr. Roux, 75015 Paris, France.

STAR Protocols
|January 31, 2025
PubMed
Summary
This summary is machine-generated.

Mouse oocytes lacking branched actin show extended cortical contractions during meiosis I. This study details a protocol for observing and analyzing these contractions using time-lapse imaging and ImageJ plugins.

Keywords:
cell culturedevelopmental biologymicroscopysingle cell

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Last Updated: May 30, 2025

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Biophysics

Background:

  • Actin dynamics are crucial for cell division.
  • Cortical contractions in oocytes regulate meiotic progression.
  • Branched actin's role in oocyte mechanics is not fully understood.

Purpose of the Study:

  • To present a protocol for observing mouse oocyte cortical contractions.
  • To analyze the impact of actin structure on meiotic division.
  • To provide a method for quantifying cortical fluctuations.

Main Methods:

  • Collection of prophase I arrested mouse oocytes.
  • Time-lapse imaging in transillumination without labeling.
  • Image analysis using ImageJ plugins (Oocytor and Radioak) for segmentation and fluctuation measurement.

Main Results:

  • Oocytes lacking branched actin exhibit prolonged cortical contractions.
  • The protocol allows for label-free observation of meiotic events.
  • Quantification of cortical fluctuations is achieved through image analysis.

Conclusions:

  • Branched actin is essential for regulating the duration of cortical contractions during mouse oocyte meiosis I.
  • This protocol facilitates the study of oocyte mechanics and cell division.
  • The findings contribute to understanding the biophysical regulation of meiosis.