A Cutting-Edge Multilayer Nanofiber Wound Dressing: Design, Synthesis, and Investigation for Enhanced Wound Healing...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Nanotechnology

Background:

• Wound healing relies heavily on effective dressing materials.
• Electrospun nanofibrous materials show potential for wound care due to their large surface area and ECM-like structure.
• Current treatments require innovation for enhanced patient outcomes.

Purpose of the Study:

• To formulate a novel three-layered nanofibrous wound dressing using electrospinning.
• To enhance patient well-being, provide antimicrobial properties, and expedite wound healing.
• To utilize polyurethane (PU), quercetin (Q)-loaded polyethylene glycol (PEG), polyvinyl alcohol (PVA), and gelatin for the dressing.

Main Methods:

• Fabrication of a three-layered nanofibrous wound dressing via electrospinning.
• Characterization using Scanning Electron Microscopy (SEM) and Fourier-Transform Infrared Spectroscopy (FT-IR).
• In vitro assessment using human cell cultures and in vivo evaluation in rat wound models.

Main Results:

• The nanofibrous dressing demonstrated anti-toxic effects on human epithelial and keratin cells.
• In vitro models showed complete wound closure within 48 hours.
• In vivo studies in rats indicated significant wound healing progress over 14 days, with gene expression analysis supporting healing mechanisms.

Conclusions:

• The developed nanofibrous wound dressing accelerates healing and possesses antibacterial properties.
• Its innovative design surpasses current treatments, offering a promising solution for wound management.
• The dressing promotes faster wound closure and supports a conducive healing environment.