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Published on: April 18, 2019
Synergistic antimicrobial activities of peroxymonosulfate with Ce-FcDC as an activator
Hui Xie1, Sihui Xie1, Li Jiang1
1College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541006, Guangxi, China.
Abstract:
Ce-MOFs with ferrocenedicarboxylic acid ligands (Ce-FcDC) as a bifunctional nanozyme exhibited high peroxidase (POD)-mimicking activity and superoxide dismutase (SOD)-mimicking activity. H2O2 was produced from catalytic hydrolysis of peroxymonosulfate (PMS) using Ce-FcDC as a catalyst. The growth of E. coli and S. aureus were synergistically and more effectively suppressed by PMS in the presence of Ce-FcDC, in comparison with the sole use of PMS or Ce-FcDc. Under the catalysis of Ce-FcDC as the POD-mimicking nanozyme, PMS could be activated by Ce-FcDC to produce SO4•- and •OH and H2O2 from the hydrolysis of PMS was further derivatized to O2•- and •OH. Ce-FcDC as the SOD-mimicking nanozyme causes O2•- to form H2O2. The generation of O2•- and •OH were confirmed using p-benzoquinone and isopropanol alcohol as the scavengers. The resulted SO4•-, O2•-, and •OH from combination of PMS with Ce-FcDC as an activator may have key roles for suppressing the growth of E. coli and S. aureus. This strategy could be an effective approach for suppressing the growth and preventing infections or pollutions of some other microbial cells as well.
Insights
Ce-FcDC nanozymes show dual peroxidase and superoxide dismutase activity, effectively suppressing bacterial growth by generating reactive oxygen species from peroxymonosulfate. This offers a novel antimicrobial strategy.
Area of Science:
- Materials Science
- Nanotechnology
- Biochemistry
Background:
- Nanozymes offer tunable catalytic properties for various applications.
- Bifunctional nanozymes with combined enzyme-mimicking activities are highly sought after.
- Controlling microbial growth is crucial in healthcare and environmental protection.
Purpose of the Study:
- To develop and characterize Ce-MOFs with ferrocenedicarboxylic acid ligands (Ce-FcDC) as a bifunctional nanozyme.
- To investigate the peroxidase (POD)-mimicking and superoxide dismutase (SOD)-mimicking activities of Ce-FcDC.
- To evaluate the efficacy of Ce-FcDC and peroxymonosulfate (PMS) in suppressing bacterial growth.
Main Methods:
- Synthesis and characterization of Ce-FcDC nanozymes.
- Assays to determine POD-mimicking and SOD-mimicking activities.
- Experiments to assess the synergistic antibacterial effect of Ce-FcDC and PMS against E. coli and S. aureus.
- Scavenger studies using p-benzoquinone and isopropanol alcohol to confirm reactive oxygen species generation.
Main Results:
- Ce-FcDC exhibited significant POD-mimicking activity, catalyzing PMS hydrolysis to produce SO₄•−, •OH, and H₂O₂.
- Ce-FcDC demonstrated SOD-mimicking activity, converting O₂•− to H₂O₂.
- The combination of Ce-FcDC and PMS synergistically inhibited the growth of E. coli and S. aureus more effectively than either alone.
- Reactive oxygen species including SO₄•−, O₂•−, and •OH were confirmed as key players in bacterial suppression.
Conclusions:
- Ce-FcDC acts as a bifunctional nanozyme with potent POD and SOD-mimicking activities.
- The synergistic action of Ce-FcDC and PMS generates a cocktail of reactive oxygen species, leading to effective bacterial growth inhibition.
- This Ce-FcDC/PMS system presents a promising strategy for combating microbial infections and pollution.
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