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Related Experiment Video

Updated: Jun 13, 2025

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

Published on: July 4, 2018

Designing and Evaluating Circadian Experiments on Mouse Skin.

Junyan Duan¹, Satya Swaroop Karri², Kiarash Forouzesh²

¹Center for Complex Biological Systems, University of California, Irvine, Irvine, California, USA; The NSF-Simons Center for Multiscale Cell Fate Research, University of California, Irvine, Irvine, California, USA.

The Journal of Investigative Dermatology

|February 1, 2025

Summary

This summary is machine-generated.

The mouse skin harbors a circadian clock regulating key functions like metabolism and immunity. This study guides researchers on experimental design for studying skin circadian rhythms and their role in health and disease.

Keywords:

Bioinformatics Circadian Skin Transgenic mouse models

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Last Updated: Jun 13, 2025

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

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Published on: July 4, 2018

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Published on: August 8, 2019

Analysis of Circadian Photoresponses in Drosophila Using Locomotor Activity

Analysis of Circadian Photoresponses in Drosophila Using Locomotor Activity

Area of Science:

Chronobiology
Dermatology
Genetics

Background:

The skin possesses a robust circadian clock influenced by light via the suprachiasmatic nucleus.
This skin clock regulates critical processes such as metabolism, stem cell activation, immune cell activity, and inflammatory responses.
Diurnally regulated genes in the skin are linked to various skin diseases, highlighting the relevance of circadian biology.

Purpose of the Study:

To provide comprehensive guidance for designing experiments investigating mouse skin circadian biology.
To emphasize the importance of considering technical and biological factors in experimental planning.
To detail methods for manipulating the skin clock and analyzing resulting data.

Main Methods:

Guidance on general circadian experimental design and mouse housing.
Recommendations for aligning sample collection with hair cycle stage and animal age.
Introduction to clock disruption methods (light/feeding schedules, transgenic models) and transcriptomic analysis (bulk and single-cell).

Main Results:

The mouse skin serves as a valuable model for understanding circadian roles in tissue health and disease.
Experimental design must account for hair cycle, age, and specific clock manipulation techniques.
Transcriptomic data analysis is crucial for elucidating circadian mechanisms in the skin.

Conclusions:

Proper experimental design is critical for studying mouse skin circadian biology.
Understanding the skin clock is essential for both maintaining tissue health and addressing skin diseases.
This work provides a framework for future research in skin chronobiology.