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Updated: Aug 19, 2026

Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos
Published on: January 30, 2013
Protocol for multiplexed RNAscope-based imaging of mRNAs in whole-mount adult Drosophila brains
Meilin Wu1, Vanessa Lambatan1, Peng Guo2
1Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093, USA.
Abstract:
Visualizing the expression of mRNAs using traditional in situ hybridization is often hampered by obstacles including weak signal, high background, and poor probe specificity. Here, we present a protocol utilizing RNAscope (ACD) to overcome these obstacles and detect multiple types of mRNAs simultaneously in whole-mount adult Drosophila brains. We further describe how mRNAs can be reliably quantified in any cells that can be targeted by common binary expression systems such as Gal4/UAS and labeled by immunohistochemistry. For complete details on the use and execution of this protocol, please refer to De et al.1.
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