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Updated: May 29, 2025
Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing
Published on: September 26, 2019
MSC-EVs and UCB-EVs promote skin wound healing and spatial transcriptome analysis
Ruonan Li1, Haotian Wang1, Xiaolong Wang2
1Key Laboratory of Animal Biochemistry and Nutrition of Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, No.15 Longzihu University Area, Zhengdong New District, Zhengzhou, 450046, China.
Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) and umbilical cord plasma-derived extracellular vesicles (UCB-EVs) promote wound healing and reduce scarring. These EVs enhance fibroblast activity and modulate signaling pathways for improved skin regeneration.
Area of Science:
- Regenerative Medicine
- Biotechnology
- Dermatology
Background:
- Extracellular vesicles (EVs) mediate regenerative effects via bioactive molecule transfer.
- Mesenchymal stem cell-derived EVs (MSC-EVs) and umbilical cord plasma-derived EVs (UCB-EVs) are potential therapeutic agents.
Purpose of the Study:
- To investigate the effects of MSC-EVs and UCB-EVs on wound healing and scar formation.
- To analyze cellular and molecular mechanisms using spatial transcriptomics.
Main Methods:
- Isolation and characterization of MSC-EVs and UCB-EVs.
- In vitro assays for fibroblast migration and proliferation.
- In vivo wound healing models in mice with histological and spatial transcriptomics analysis.
Main Results:
- MSC-EVs and UCB-EVs promoted human dermal fibroblast proliferation and migration in vitro.
- In vivo, these EVs accelerated wound closure and reduced scar width.
- Spatial transcriptomics revealed modulation of myofibroblast markers, TGF-β, and Wnt signaling pathways.
Conclusions:
- MSC-EVs and UCB-EVs enhance skin wound healing and reduce scarring.
- These EVs represent promising therapeutic agents for regenerative skin treatments.
