Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

tRNA-m1A Modification Safeguards Fetal Liver HSPCs from DNA Damage via Maintaining Iron Homeostasis.

Blood·2026

Same author

Quantitative analysis of small RNA pseudouridylation reveals interplay of PUS enzymes in tRNA anticodon stem-loop.

Nature communications·2026

Same author

Single-strand deaminase-assisted editing for functional RNA manipulation.

Nature biotechnology·2026

Same author

CRISPR-free RNA base editing mediated PTC-readthrough restores hearing in mice with Otof nonsense mutation.

Nature communications·2025

Same author

Pt-seq unveils the genomic binding pattern of platinum-based drugs.

Science advances·2025

Same author

Advancing DNA and RNA Modification Detection via Nanopore Sequencing.

ACS nano·2025

Search research articles

Related Experiment Video

Updated: May 29, 2025

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study

Published on: July 7, 2023

Protocol for profiling RNA m⁵C methylation at base resolution using m⁵C-TAC-seq.

Xiaoting Zhang¹, Liang Lu², Chengqi Yi³

¹State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China.

|February 2, 2025

Summary

This summary is machine-generated.

We developed a new method, m5C-TAC-seq, for precise transcriptome-wide RNA 5-methylcytosine (m5C) profiling. This bisulfite-free approach accurately maps m5C modifications across various RNA types at base resolution.

Keywords:

Bioinformatics Genetics Genomics Molecular Biology Molecular/Chemical Probes RNAseq Sequence analysis Sequencing

More Related Videos

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

Related Experiment Videos

Last Updated: May 29, 2025

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study

Published on: July 7, 2023

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

Area of Science:

Epigenetics
RNA Biology
Molecular Biology

Background:

RNA 5-methylcytosine (m5C) is a prevalent epitranscriptomic modification.
m5C plays a significant role in regulating gene expression.
Accurate profiling of m5C modifications is essential for understanding its functional impact.

Purpose of the Study:

To present a novel protocol for transcriptome-wide m5C methylome profiling.
To introduce a bisulfite-free detection strategy for m5C modifications.
To enable base-resolution mapping of m5C using a specific sequencing technique.

Main Methods:

Developed ten-eleven translocation (TET)-assisted chemical labeling sequencing (m5C-TAC-seq).
Detailed protocol steps for TET-assisted chemical labeling.
Included procedures for library construction and data analysis.

Main Results:

m5C-TAC-seq enables accurate and robust detection of m5C modifications.
The protocol allows for transcriptome-wide methylome profiling at base resolution.
The method is applicable to various RNA species.

Conclusions:

m5C-TAC-seq provides a reliable and efficient method for m5C profiling.
This technique advances the study of RNA modifications and gene regulation.
The protocol facilitates deeper investigation into the functional roles of m5C.