Characterization and antioxidant activity of differentiated fractionation lignin from corn stover
Shuai Yuan1, Boyu Li1, Liya Chang1
1College of Pharmacy, Qiqihar Medical University, Qiqihar, PR China.
Abstract:
Lignin contains many chemical functional groups with multiple biological activities. However, the heterogeneity of lignin such as complex structure and high polydispersity, and poor dissolution performance hinders its value-added application. In this study, it was found that there was a significant difference in the solubility of the chemical components of corn stover alkali-extracted lignin-carbohydrate complex (CSALCC) in a 0.6 M NaHCO3 solution. Herein, CSALCC was fractionated using a 0.6 M NaHCO3 solution, hot water, acid precipitation, and macroporous adsorption resin D101 column chromatography to afford fraction F1-1, F2, and F3-1. To demonstrate the improvement in composition, water solubility and antioxidant activity of the fractions. The characterization techniques UV, FTIR, NMR, TGA, SDS-PAGE and GPC were employed. Antioxidant activities were evaluated by ABTS, ORAC and ferric reducing power assay. F1-1 consists mainly of hemicellulose and is soluble in deionized water. F2 is a more water-soluble lignin than CSALCC, which is conducive to the development of value-added products of lignin in aqueous systems. F3-1 was acidic-soluble lignin with the highest total polyphenol content of all fractions, and exhibited higher water solubility, antioxidant properties and UV absorption. F3-1 may have potential application in cosmetics, pharmaceuticals, the food processing field.
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