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Enzyme linked immunosorbent assay for human apolipoprotein C-III
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|February 1, 1985
Summary
A new sandwich enzyme-linked immunosorbent assay (ELISA) accurately measures apolipoprotein C-III levels in plasma. This sensitive assay provides reliable quantification for research and clinical applications.
Area of Science:
- Biochemistry
- Immunology
- Clinical Chemistry
Background:
- Apolipoprotein C-III (ApoC-III) is a key regulator of triglyceride metabolism.
- Accurate quantification of ApoC-III is crucial for understanding dyslipidemias and cardiovascular disease risk.
Purpose of the Study:
- To develop and validate a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) for quantifying apolipoprotein C-III.
Main Methods:
- Development of a sandwich ELISA using specific anti-apolipoprotein C-III antibodies for plate coating and enzyme conjugation.
- Optimization of assay conditions to determine sensitivity and working range.
- Validation of the assay by comparing results with an established immunonephelometric method.
Main Results:
- The developed ELISA demonstrated high sensitivity (0.3 ng) with a working range of 1-6 ng of apolipoprotein C-III.
- Assay performance was consistent across purified protein, plasma, and lipoprotein fractions, unaffected by delipidation.
- Excellent correlation (r=0.982) was observed when compared to immunonephelometry, with low intra-assay (3.6%) and inter-assay (4.2%) coefficients of variation.
Conclusions:
- A robust and sensitive sandwich ELISA for apolipoprotein C-III has been successfully developed.
- This assay offers a reliable method for apolipoprotein C-III quantification in biological samples.
- The validated ELISA is suitable for research applications in lipid metabolism and cardiovascular disease.