Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
Ribozymes02:47

Ribozymes

11.1K
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
11.1K
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

7.1K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
7.1K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

13.1K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TRNAU1AP and PRPF39 establish integrated control over processing of most abundant human non-coding RNAs.

Nature communications·2026
Same author

Identification of RMP24 and RMP64, human ribonuclease MRP-specific protein components.

Cell reports·2025
Same author

Identification of human pathways acting on nuclear non-coding RNAs using the Mirror forward genetic approach.

Nature communications·2025
Same author

Identification of Human Pathways Acting on Nuclear Non-Coding RNAs Using the Mirror Forward Genetic Approach.

bioRxiv : the preprint server for biology·2024
Same author

Prenatal ultrasound findings regarding obstruction to blood flow and an umbilical artery varix caused by umbilical cord torsion.

European journal of obstetrics, gynecology, and reproductive biology·2022
Same author

L-arginine protects cementoblasts against hypoxia-induced apoptosis through Sirt1-enhanced autophagy.

Journal of periodontology·2021
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: May 29, 2025

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

27.5K

Identification of Two Elusive Human Ribonuclease MRP-Specific Protein Components.

Rui Che1,2, Bhoomi Mirani1,2, Monireh Panah1,2

  • 1Dept. of Genetics and Biochemistry, Clemson University, Clemson, SC 29631, USA.

Biorxiv : the Preprint Server for Biology
|February 3, 2025
PubMed
Summary
This summary is machine-generated.

Researchers identified two new proteins, RPP24 and RPP64, essential for human Ribonuclease MRP (RNase MRP) function in pre-ribosomal RNA processing. These proteins are unique to RNase MRP and not involved in Ribonuclease P (RNase P) activity.

More Related Videos

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

8.9K
An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
09:45

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

11.0K

Related Experiment Videos

Last Updated: May 29, 2025

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

27.5K
Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

8.9K
An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
09:45

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

11.0K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Ribonuclease MRP (RNase MRP) and Ribonuclease P (RNase P) are crucial human ribonucleoprotein enzymes with shared protein components.
  • RNase MRP processes pre-ribosomal RNA (pre-rRNA) at ITS1 site 2, while RNase P cleaves pre-tRNA 5' leader sequences.
  • The specific protein constituents unique to human RNase MRP have remained largely uncharacterized.

Purpose of the Study:

  • To identify novel protein components specific to human nuclear RNase MRP.
  • To investigate the functional roles of newly identified proteins in pre-rRNA processing.
  • To differentiate the protein composition of RNase MRP from RNase P.

Main Methods:

  • Genome-wide forward genetic screening was employed to identify essential genes.
  • Functional assays were performed to assess the role of identified proteins in pre-rRNA processing.
  • Co-immunoprecipitation and structural predictions were used to analyze protein-RNA interactions and structural similarities.

Main Results:

  • Two previously poorly characterized human genes, RPP24 and RPP64, were identified and found to be essential for pre-rRNA ITS1 site 2 processing.
  • RPP24 and RPP64 proteins associate with RNA MRP but are not required for RNase P activity or association with RNA H1.
  • RPP24 and RPP64 show predicted structural similarities to yeast RNase MRP-specific components, indicating conserved structural tetapi divergent functional roles.

Conclusions:

  • RPP24 and RPP64 are the first identified protein components unique to human nuclear RNase MRP.
  • These findings expand our understanding of the distinct protein machinery of RNase MRP and RNase P.
  • The unique proteins contribute to the specific function of RNase MRP in pre-rRNA maturation.