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Related Experiment Video

Updated: May 29, 2025

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Imaging cell spheroid clusters: An MRI protocol for non-invasive standardized characterization.

Rebecca Wißmann1, Petros Martirosian1,2, Marina Danalache3

  • 1Department of Diagnostic and Interventional Radiology, University Hospital of Tübingen, D-72076, Tübingen, Germany.

Heliyon
|February 3, 2025
PubMed
Summary

We developed a non-invasive magnetic resonance imaging (MRI) method to assess stem cell and cancer spheroids. This technique allows for the visualization of spheroid composition and vitality without damaging micro-structures.

Keywords:
3D cultureExtracellular matrixMagnetic resonance imagingMesenchymal stem cellsMicroenvironmentRegenerative medicineSpheroidsTumor modelVisualization

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Medical Imaging

Background:

  • Three-dimensional (3D) cell cultures, such as spheroids, serve as advanced models bridging in vitro and in vivo research.
  • Spheroids exhibit natural cell-cell interactions and extracellular matrix development, mimicking in vivo environments.
  • Conventional assessment methods for cell spheroids are destructive, hindering micro-structural analysis.

Purpose of the Study:

  • To develop and propose a non-invasive method for assessing cell spheroids using magnetic resonance imaging (MRI).
  • To enable the study of spheroid composition, vitality, and micro-environment without compromising structural integrity.
  • To create an MRI-compatible setup for culturing and analyzing cell spheroids using standard clinical MRI systems.

Main Methods:

  • Development of an MRI-compatible setup for culturing cell spheroids.
  • Utilizing standard 3 Tesla (3T) whole-body MRI systems for non-invasive imaging.
  • Conducting systematic studies with various cell types to validate the approach.

Main Results:

  • Demonstrated the capability of MRI to provide information on spheroid composition and vitality non-invasively.
  • Successfully developed an MRI-compatible culturing system for cell spheroids.
  • Showcased the potential for simultaneous actuation and visualization of cell spheroids.

Conclusions:

  • Non-invasive MRI offers a valuable tool for analyzing cell spheroids, overcoming limitations of destructive conventional methods.
  • The developed MRI-compatible setup and approach are suitable for standard clinical MRI systems.
  • This technique holds broad potential for diverse applications in cell biology and regenerative medicine research.