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Related Experiment Video

Updated: May 29, 2025

Surgical Method for Virally Mediated Gene Delivery to the Mouse Inner Ear through the Round Window Membrane

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Limiting Hearing Loss in Transgenic Mouse Models.

Travis A Babola¹, Naomi Donovan¹, Sean S Darcy¹

¹Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21205.

|February 3, 2025

Summary

Transgenic mice on a C57BL/6 background often lose hearing, complicating research. Crossing them with B6.CAST-Cdh23+ mice preserves hearing, enabling clearer systems neuroscience studies.

Keywords:

behavior deaf hearing loss mouse transgenic

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Last Updated: May 29, 2025

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Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice

Published on: May 10, 2019

Area of Science:

Neuroscience
Genetics
Auditory Systems

Background:

Transgenic mice are crucial for neural circuit research.
C57BL/6 mice exhibit progressive hearing loss, confounding auditory cortex studies.
Outbreeding mice limits hearing loss but introduces variability and genetic complexity.

Purpose of the Study:

To develop a method for preserving hearing in transgenic mice for improved neuroscience research.
To investigate the impact of hearing loss on auditory cortex function in C57BL/6 mice.
To establish a cost-effective approach for generating complex genotypes with preserved hearing.

Main Methods:

Crossing transgenic mice with congenic B6.CAST-Cdh23+ mice.
Utilizing widefield and two-photon imaging to analyze the auditory cortex.
Assessing neural responses to various sound frequencies and levels in adult mice.

Main Results:

Standard C57BL/6 mice showed elevated hearing thresholds and cortical reorganization.
Congenic B6.CAST-Cdh23+ crossed mice (Ahl+ C57BL/6) maintained low hearing thresholds into adulthood.
Ahl+ C57BL/6 mice demonstrated robust neural responses across tested auditory parameters.

Conclusions:

Crossing with B6.CAST-Cdh23+ mice effectively preserves hearing in transgenic mice.
This method facilitates more interpretable systems neuroscience research by mitigating hearing loss artifacts.
The approach provides a cost-effective strategy for creating complex transgenic genotypes with preserved auditory function.