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Related Concept Videos

Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Related Experiment Video

Updated: May 29, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

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Spatial-Omics Methods and Applications.

Arutha Kulasinghe1,2, Naomi Berrell2, Meg L Donovan2

  • 1Frazer Institute, Faculty of Medicine, University of Queensland, Brisbane, QLD, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|February 3, 2025
PubMed
Summary
This summary is machine-generated.

Spatial omics technologies now allow researchers to study tissues in situ, preserving anatomical context. This field has advanced rapidly but still faces challenges in standardization, throughput, and ease of use.

Keywords:
Digital pathologySpatial biologySpatial proteomicsSpatial transcriptomicsSpatial-omics

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genomics

Background:

  • Traditional tissue profiling methods like bulk and single-cell analyses have historically omitted spatial context.
  • The emergence of spatial technologies over the past five years has revolutionized tissue analysis by enabling in situ investigation of proteins and transcripts.
  • Spatial omics preserves tissue anatomy and histology, offering unprecedented insights into microenvironments.

Purpose of the Study:

  • To provide a comprehensive overview of the current landscape of spatial omics technologies.
  • To highlight key technological advancements and applications in the field of spatial omics.
  • To identify and discuss the existing challenges and limitations in spatial omics methodologies.

Main Methods:

  • Spatial omics technologies enable highly multiplexed analysis of biomolecules (e.g., RNAs, proteins) within their native spatial context.
  • These methods allow for in situ investigation of tissue samples, maintaining anatomical and histological integrity.
  • The chapter reviews current technological approaches and their capabilities.

Main Results:

  • Spatial omics has matured into a thriving field with broad applications spanning basic, translational, and clinical research.
  • Significant technological advances have been made, enabling detailed spatial profiling of tissues.
  • Widespread adoption of spatial technologies is evident across various research domains.

Conclusions:

  • Spatial omics represents a paradigm shift in biological research, integrating molecular data with spatial information.
  • Despite rapid progress and adoption, challenges in standardization, sample compatibility, throughput, resolution, and usability persist.
  • Continued development is crucial to fully realize the potential of spatial omics in advancing biological and medical sciences.