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Tissue-friendly dentin treatments as a potential element in revascularization protocol (ex-vivo study)
Hala Fayek Khalil1, Nadia Ezz El Din Metwalli2, Sara Magdy3
1Department of Endodontics, Faculty of Dentistry, The British University in Egypt, El Shorouk City, PO Box 43Suez Road, Cairo, 11837, Egypt. hala.fayek@bue.edu.eg.
BMC Oral Health
|February 3, 2025
Summary
Dual Rinse HEDP and curcumin show promise as tissue-friendly endodontic irrigants. Dual Rinse HEDP demonstrated superior cell adherence and osteogenic differentiation, supporting its use in regenerative endodontics.
Area of Science:
- Biomaterials Science
- Endodontics
- Regenerative Medicine
Background:
- Endodontic treatment requires effective yet safe irrigants for pulp tissue and microbial elimination.
- Sodium hypochlorite, while standard, exhibits cytotoxicity, necessitating exploration of alternatives for revitalization and regeneration.
- Investigating novel irrigants like Dual Rinse HEDP and curcumin is crucial for advancing endodontic protocols.
Purpose of the Study:
- To evaluate the cytotoxicity, cell proliferation, dentin adhesion, and osteogenic differentiation of Dual Rinse HEDP and curcumin compared to sodium hypochlorite.
- To assess the potential of these irrigants in revitalization and regenerative endodontic procedures.
- To determine the optimal exposure times (1, 5, and 15 minutes) for irrigant efficacy and safety.
Main Methods:
- Cytotoxicity assessed via MTT assay; cell proliferation evaluated using Trypan blue exclusion.
- Cell adherence to dentin analyzed by scanning electron microscopy (SEM).
- Osteogenic differentiation evaluated through alizarin red staining and gene expression analysis (BMP-2, TGF-β1, VEGF, DSPP).
Main Results:
- Dual Rinse HEDP exhibited the lowest cytotoxicity at 1 and 5 minutes and the highest cell proliferation.
- HEDP significantly upregulated osteogenic gene expression (BMP-2, TGF-β1, VEGF, DSPP) and promoted calcium deposition.
- Curcumin showed moderate osteogenic potential, while sodium hypochlorite demonstrated higher cytotoxicity.
Conclusions:
- Dual Rinse HEDP and curcumin are biocompatible irrigants suitable for endodontic applications.
- Dual Rinse HEDP enhances stem cell adherence to dentin and promotes osteogenic differentiation.
- Dual Rinse HEDP shows significant potential for use in regenerative endodontic protocols.
