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Related Concept Videos

Oligosaccharide Assembly01:24

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

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A Quantitative Glycomics and Proteomics Combined Purification Strategy
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Nanofiltration-based purification process for whole-cell transformed prebiotic galactooligosaccharides.

Anita Srivastava1,2, Arjun Rastogi3, Avijeet S Jaswal3

  • 1Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz-Khas, New-Delhi, 110016, India. ird600137@iitd.ac.in.

Bioprocess and Biosystems Engineering
|February 4, 2025
PubMed
Summary

This study optimized galactooligosaccharides (GOS) enrichment using nanofiltration, achieving 88.8% recovery. The nano-filtered GOS effectively supports microbial growth, highlighting its prebiotic potential.

Keywords:
FermentationGalactooligosaccharidesGalactosidaseNanofiltrationPermeabilized cells

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Area of Science:

  • Biotechnology
  • Food Science
  • Separation Science

Background:

  • Galactooligosaccharides (GOS) are valuable prebiotics.
  • Current methods for GOS enrichment face challenges with purity and yield.
  • Kluyveromyces marxianus 3551 is a microbial source for GOS synthesis.

Purpose of the Study:

  • To investigate the enrichment of galactooligosaccharides (GOS) synthesized by Kluyveromyces marxianus 3551.
  • To optimize GOS enrichment using nanofiltration technology.
  • To evaluate the prebiotic potential of the enriched GOS.

Main Methods:

  • Synthesis of GOS using whole cells of Kluyveromyces marxianus 3551 in a bioreactor.
  • Fractionation of the synthesized sugar mixture using three different spiral wound nanofiltration membranes (polysulfone, cellulose acetate, polyethersulfone).
  • Optimization of nanofiltration conditions (temperature, transmembrane pressure) using the best-performing membrane (polyethersulfone, 400-1000 Da cut-off).
  • Analysis of sugar concentrations via high-performance liquid chromatography (HPLC).
  • Microbial growth assays to assess prebiotic effectiveness.

Main Results:

  • The polyethersulfone membrane (NFPES-03) demonstrated superior performance in fractionating the GOS mixture.
  • Optimal GOS enrichment was achieved at 50 °C and 15 bar transmembrane pressure, maximizing trisaccharide and disaccharide/monosaccharide rejection.
  • The integrated process achieved an 88.8% recovery of GOS.
  • Nano-filtered GOS supported the growth of various microbes, confirming its prebiotic properties.

Conclusions:

  • Nanofiltration, particularly with a polyethersulfone membrane, is an effective method for enriching galactooligosaccharides (GOS).
  • Optimized conditions enhance GOS purity and recovery.
  • The enriched GOS serves as a potent prebiotic source, validated by microbial growth studies.