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Related Concept Videos

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Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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Aggregation-induced emission-based fluorescent probes for cellular microenvironment detection.

Xiang-Fei Li1, Fu-Gen Wu1

  • 1State Key Laboratory of Digital Medical Engineering, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, 2 Southeast University Road, Nanjing, 211189, China.

Biosensors & Bioelectronics
|February 4, 2025
PubMed
Summary
This summary is machine-generated.

Aggregation-induced emission (AIE) fluorescent molecules offer advanced imaging for cellular microenvironments. This review highlights their progress in detecting key parameters like pH, viscosity, and biomolecules for disease insights.

Keywords:
Aggregation-induced emissionCell imagingCellular microenvironmentDetectionFluorescent probes

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Area of Science:

  • Biomedical Engineering
  • Chemical Biology
  • Molecular Imaging

Background:

  • The cellular microenvironment critically influences cell behavior and disease pathogenesis.
  • Dynamic monitoring of the microenvironment aids in disease risk prediction and progression tracking.
  • Aberrant cellular microenvironments are linked to various diseases.

Purpose of the Study:

  • To review recent advancements in using aggregation-induced emission (AIE) fluorescent molecules for cellular microenvironment detection.
  • To highlight the application of AIE probes in imaging and sensing key microenvironmental parameters and biological components.
  • To discuss design principles, mechanisms, performance, and future prospects of AIE probes.

Main Methods:

  • Focus on AIE fluorescent probes for sensing cellular microenvironment parameters.
  • Analysis of probes for detecting pH, viscosity, polarity, temperature, gas molecules, metal ions, redox state, and proteins.
  • Review of probe design, detection mechanisms, sensing performance, and biological applications.

Main Results:

  • AIE molecules show unique characteristics advantageous for cellular microenvironment imaging and sensing.
  • Significant progress has been made in developing AIE probes for various microenvironmental analytes.
  • AIE probes enable precise detection of key parameters and biological components within the cellular milieu.

Conclusions:

  • AIE fluorescent probes are powerful tools for investigating cellular microenvironments.
  • Continued development of AIE probes promises enhanced sensitivity and specificity for biological detection.
  • This field holds significant potential for advancing disease diagnostics and understanding biological processes.