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Updated: May 29, 2025
The 4-vessel Sampling Approach to Integrative Studies of Human Placental Physiology In Vivo
Published on: August 2, 2017
"Optimal timing for RNA isolation: Recommendations for placenta sample collection under clinical conditions"
J Sommer1, L Ehrlich1, A Kühn2
1Clinic of Obstetrics, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin Institute of Health, Germany; Division of Experimental Obstetrics, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin Institute of Health, Germany.
Abstract:
In placenta research, tissue quality is essential for following laboratory investigations. In clinical settings with deliveries as an unpredictable factor, delayed sampling or incorrect storage until sampling is a common problem. Therefore, we set out to define time points for different storage conditions where good tissue quality is still achievable. RNA related parameters like 260/280-ratio, RNA integrity number (RIN) and gene expression were analyzed in 172 samples over a 24-h interval. We suggest a cut-off-point of 3-h after delivery of the placenta to ensure good tissue and RNA quality, especially when selecting genes with complex or low mRNA expression levels.
