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Deuterated oxazines are bright near-infrared fluorophores for mitochondrial imaging and single molecule spectroscopy

Blaise Gatin-Fraudet1, Julius Seifert1, Kris Sarach1

  • 1Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Robert-Rössle-Str. 10, 13125 Berlin, Germany. gatinfraudet@fmp-berlin.de.

Chemical Communications (Cambridge, England)
|February 6, 2025
PubMed

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¹H NMR of Labile Protons: Deuterium (²H) Substitution00:48

¹H NMR of Labile Protons: Deuterium (²H) Substitution

This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.