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Updated: May 29, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

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Genome sequences of Klebsiella pneumoniae bacteriophages SF_KL2 and SF_KL25

Sophia Lorenz1,2, Gabriel Abreu1,2, Hugo Oliveira1,2

  • 1CEB-Centre of Biological Engineering, University of Minho, Braga, Portugal.

Microbiology Resource Announcements
|February 6, 2025
PubMed

Insights

This study details the genomes of two Klebsiella pneumoniae phages, SF_KL2 and SF_KL25. These phages infect multidrug-resistant Klebsiella pneumoniae, offering potential in combating hospital-acquired infections.

Area of Science:

  • Microbiology
  • Genomics
  • Virology

Background:

  • Klebsiella pneumoniae is a significant cause of nosocomial infections.
  • Multidrug-resistant strains of K. pneumoniae pose a growing public health threat.
  • Bacteriophages are viruses that infect bacteria and are being explored as therapeutic agents.

Purpose of the Study:

  • To characterize the genomes of two Klebsiella pneumoniae phages, SF_KL2 and SF_KL25.
  • To provide genomic data for phages infecting multidrug-resistant K. pneumoniae.
  • To contribute to the understanding of Webervirus genus phages.

Main Methods:

  • Isolation of bacteriophages from wastewater samples.
  • Genome sequencing and analysis of isolated phages.
  • Phylogenetic analysis to determine phage classification.

Main Results:

  • The genomes of phages SF_KL2 and SF_KL25 were sequenced, measuring 48,737 bp and 49,170 bp, respectively.
  • Both phages were classified within the Webervirus genus, exhibiting a siphovirus morphotype.
  • The phages were isolated from wastewater in northern Portugal.

Conclusions:

  • The genomic characterization of SF_KL2 and SF_KL25 provides valuable data for phage therapy development against K. pneumoniae.
  • Understanding these phages contributes to the knowledge of the Webervirus genus and phage-bacterial interactions.
  • The isolation source highlights the potential of environmental sources for discovering novel therapeutic phages.
Keywords:
Klebsiella pneumoniaebacteriophagescapsule typesdepolymerasesgenome

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