The FRET-Based APTA Sensor/Cy3 Complex for Glucose Determination.

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Nanotechnology

Background:

• Accurate blood glucose monitoring is crucial for diabetes management.
• Existing methods may lack sensitivity, cost-effectiveness, or specificity.
• Förster Resonance Energy Transfer (FRET) offers a sensitive detection mechanism.

Purpose of the Study:

• To develop a sensitive and cost-effective fluorescent probe for blood glucose monitoring.
• To utilize a FRET-based system incorporating aptamers and quantum dots.
• To evaluate the probe's performance in biological samples.

Main Methods:

• Development of a FRET sensor using Cadmium Telluride-Thioglycolic Acid Quantum Dots (CdTe-TGA QDs) and a Cy3-labeled aptamer.
• Investigation of fluorescence quenching by glucose molecules.
• Characterization using UV-Vis, FT-IR, XPS, FESEM, EDX, TEM, AFM, and DLS.

Main Results:

• The probe achieved a low detection limit of 7.72 × 10^-9 M for glucose.
• Demonstrated excellent selectivity and specificity for glucose.
• Showed high recovery rates (96.00-101.11%) in human serum and urine.

Conclusions:

• The developed FRET-based APTA sensor/Cy3 complex is a sensitive and reliable tool for glucose detection.
• This probe has potential for clinical diagnostics and biological metabolite screening.
• The study highlights the utility of quantum dot-aptamer conjugates in biosensing applications.