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Related Concept Videos

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

366
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
366

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Related Experiment Video

Updated: May 29, 2025

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
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Improved real-world UHPLC-MS/MS iohexol analysis; thawed samples, improved calibration curve, and additional

A Mireille A Wessels1, Lenneke A T Junier1, Daan Touw1,2

  • 1Department of Clinical Pharmacy & Pharmacology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.

Bioanalysis
|February 7, 2025
PubMed
Summary

This study enhances iohexol measurement for glomerular filtration rate (GFR) by improving sample stability and validation. The optimized method allows for reliable analysis of iohexol in various matrices, ensuring accurate GFR assessment in clinical settings.

Keywords:
IohexolUHPLC-MS/MSextended stabilitymatrix comparisonreal-worldthawed samplestwo-point calibration

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Area of Science:

  • Analytical Chemistry
  • Clinical Chemistry
  • Pharmacokinetics

Background:

  • Plasma clearance of iohexol is a key method for assessing glomerular filtration rate (GFR).
  • Existing UHPLC-MS/MS methods require improved validation for real-world sample handling, including thawing and analysis in diverse matrices.
  • Enhanced stability and matrix validation are crucial for reliable iohexol determination.

Purpose of the Study:

  • To improve an existing UHPLC-MS/MS method for iohexol determination in plasma.
  • To conduct enhanced stability testing under various storage conditions.
  • To optimize calibration curves and perform partial validation in additional biological matrices.

Main Methods:

  • Stability testing of iohexol in plasma up to 9 weeks at temperatures ranging from room temperature to 37°C with intermittent 55°C exposure.
  • Comparison of eight-point versus two-point calibration curves for iohexol quantification.
  • Partial validation of the method in serum, heparin plasma, urine, EDTA whole blood, and heparin whole blood.

Main Results:

  • The improved method demonstrated compliance with US FDA and European Medicines Agency bioanalytical guidelines.
  • Human EDTA plasma samples showed stability for up to 9 weeks under tested conditions.
  • The method is partially validated for multiple biological matrices, including serum and whole blood.

Conclusions:

  • The optimized UHPLC-MS/MS method provides enhanced stability for iohexol in human plasma.
  • A two-point calibration curve is sufficient for accurate iohexol analysis.
  • The method's partial validation across various matrices supports its broader application in GFR assessment.