Acoustic Transmitted Decellularized Fish Bladder for Tympanic Membrane Regeneration.

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Otolaryngology

Background:

• Tympanic membrane (TM) repair requires advanced tissue-engineered membranes.
• Existing methods face challenges in biocompatibility, mechanical support, and anti-inflammatory properties.

Purpose of the Study:

• To develop and evaluate a novel decellularized fish swim bladder (DFB) scaffold loaded with mesenchymal stem cells (MSCs) for tympanic membrane perforation (TMP) repair.
• To assess the scaffold's biocompatibility, mechanical properties, and therapeutic effects in promoting TM regeneration and function.

Main Methods:

• Decellularization of fish swim bladders to create DFB scaffolds, preserving collagen structure.
• Loading DFB scaffolds with mesenchymal stem cells (MSCs) to create the DFB@MSCs system.
• In vivo evaluation of DFB@MSCs scaffolds for TMP repair, assessing inflammation, angiogenesis, TM regeneration, and hearing improvement.

Main Results:

• DFB scaffolds provide a porous, mechanically supportive microenvironment for cell growth.
• DFB@MSCs exhibit significant anti-fibrotic and anti-inflammatory effects.
• In vivo studies demonstrated reduced inflammation, enhanced angiogenesis, successful TM repair, and improved hearing.

Conclusions:

• The DFB@MSCs system is a promising biomaterial for tympanic membrane repair.
• This novel stent offers biocompatibility, mechanical support, and therapeutic benefits for TMP.
• The DFB@MSCs stent shows potential for clinical application in TM reconstruction and hearing restoration.