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Combinatorial Library-Screened Array for Rapid Clinical Sperm Quality Assessment.

Hui Huang1,2, Xiao Shi3, Weiwei Ni1

  • 1State Key Laboratory of Natural Medicines, College of Engineering, China Pharmaceutical University, Nanjing 210009, China.

Analytical Chemistry
|February 8, 2025
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Summary

This study introduces a novel sensor array strategy using combinatorial chemistry for rapid disease diagnosis. The method efficiently identifies abnormal sperm samples with high accuracy, offering a new diagnostic tool.

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Area of Science:

  • Biomedical Engineering
  • Chemical Biology
  • Diagnostics

Background:

  • Nonspecific interactions are crucial in biology but underutilized in clinical diagnostics.
  • Existing sensor arrays show promise but struggle with complex biospecimens.
  • Developing generic, efficient strategies for sensor array construction is a significant challenge.

Purpose of the Study:

  • To develop a universal strategy for constructing effective sensor arrays using combinatorial chemistry.
  • To demonstrate the practical application of this strategy in clinical diagnostics.
  • To address the lack of rapid detection techniques for sperm quality assessment.

Main Methods:

  • Employed a combinatorial chemistry-screened array strategy utilizing four-component Ugi reactions for rapid synthesis of diverse sensing elements.
  • Synthesized a library of 192 structurally diverse sensor elements.
  • Screened sensors against diverse analytes to build effective sensor arrays.

Main Results:

  • Successfully constructed a 14-element sensor array from an initial library of 192 elements.
  • Achieved 94.2% accuracy in distinguishing healthy individuals from four types of abnormal sperm samples within 1 minute.
  • Demonstrated the array's practical applicability in clinical sperm quality assessment.

Conclusions:

  • The combinatorial chemistry-screened array strategy offers a universal and efficient approach to sensor array construction.
  • This method simplifies sensor design and accelerates the development of diagnostic tools.
  • The developed array provides a rapid and accurate method for clinical sperm quality assessment.