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Related Concept Videos

Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care01:30

Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care

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A healthcare provider can diagnose a urinary tract infection (UTI) through several methods:Medical History and Symptoms: The provider will take a detailed medical history and ask about symptoms such as frequent urination, burning sensation during urination, and lower abdominal pain.Urinalysis: A clean-catch urine sample is collected in a sterile container and tested for the presence of bacteria, white blood cells (leukocytes), nitrites, blood, and protein. The presence of leukocytes and...
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Urinary tract infections (UTIs) impact various parts of the urinary system, including the kidneys, ureters, bladder, and urethra. These infections are generally bacterial, with Escherichia coli being the most common causative agent, often originating from the gastrointestinal tract. However, other bacteria, such as Staphylococcus saprophyticus, Klebsiella pneumoniae, and Proteus mirabilis, are also known to cause UTIs. The type, location, and underlying complexity of the UTI guide both...
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The pathophysiology of urinary tract infections (UTIs) encompasses several progressive stages, beginning with bacterial colonization and culminating in potential systemic complications if untreated. UTIs are primarily initiated by bacteria, such as Escherichia coli, which often originate from the gastrointestinal tract and migrate to the urinary system through the periurethral area. This migration can occur via several routes, including improper hygiene practices, sexual activity, or...
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Boosting Array-based Diagnosis of Urinary Tract Infections via a Heterobifunctional Bridge-Driven Strategy.

Yongbin Kuang1, Weiwei Ni1, Junwei Wu1

  • 1State Key Laboratory of Natural Medicines, National R&D Center for Chinese Herbal Medicine Processing, School of Engineering, China Pharmaceutical University, Nanjing, 211198, China.

Angewandte Chemie (International Ed. in English)
|December 22, 2025
PubMed
Summary

A new dual-mode sensor array offers rapid and precise diagnosis for urinary tract infections (UTIs). This advanced array identifies multiple bacteria in urine with high accuracy, paving the way for improved clinical diagnostics.

Keywords:
Bacteria identificationDual‐modal responseFluorescenceNoncovalent interactionsSensor arrays

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Microbiology

Background:

  • Array-based sensing shows promise for high-throughput detection but struggles with sensitivity in complex biological samples.
  • Clinical translation of current sensing platforms is limited by sensitivity issues in biofluids like urine.

Purpose of the Study:

  • To develop a novel heterobifunctional bridge-driven (HB-Bridge) strategy for constructing a dual-mode sensor array.
  • To enhance sensitivity and enable simultaneous detection of multiple uropathogens for rapid UTI diagnosis.

Main Methods:

  • Constructed a sensor array using HB-Bridge strategy with asymmetric termini for dual-mode sensing (covalent and electrostatic interactions).
  • Utilized perylene diimide fluorophores (PDIs) for fluorescence turn-on and quenching detection mechanisms.
  • Employed machine learning classifiers, including multilayer perceptron (MLP), for data analysis and bacterial identification.

Main Results:

  • The optimized array achieved 84.52% accuracy in identifying 21 uropathogens in urine.
  • Ultrasensitive detection of Escherichia coli with a limit of OD600 = 0.0000417.
  • MLP achieved 98.50% accuracy in distinguishing bacterial types within 30 min in a double-blind study of 150 samples, maintaining 97.92% accuracy in polymicrobial cases.

Conclusions:

  • The developed dual-mode sensing platform enables rapid and precise diagnosis of UTIs.
  • The HB-Bridge strategy offers a robust approach for sensitive and multiplexed detection in complex biofluids.
  • This technology holds significant potential for clinical translation in diagnosing urinary tract infections.