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Protocol to study the impact of breast cancer on colonization resistance of mouse microbiota using network node

Ana Laura Cano-Argüelles¹, Alejandra Wu-Chuang², Lourdes Mateos-Hernandez²

¹Parasitology Laboratory, Institute of Natural Resources and Agrobiology of Salamanca (IRNASA, CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, Spain.

|February 8, 2025

Abstract:

Network analysis is a powerful tool for investigating complex interactions between different microbial taxa within a community. We present a protocol to study the gut microbial community in a mouse model of breast cancer using a network-based approach. Here, we describe the procedures for tumor cell production and inoculation and 16S rRNA data processing. We then detail steps for constructing co-occurrence networks based on correlations between microbial abundances. For complete details on the use and execution of this protocol, please refer to Wu-Chuang et al.¹.

Keywords:

Cancer Microbiology Model Organisms Systems biology

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